02998nas a2200361   4500008004100000022004200041245010600083210006900189250001000258260001000268300001400278490000700292520175200299653004702051653001902098653001002117653003302127653002602160653001102186653006202197653005702259653001102316653004602327653004502373653004502418100002402463700002002487700001802507700002802525700002002553700001902573856004402592       2003                        eng d  a0041-1337 (Print) 0041-1337 (Linking)00aHuman T-cell leukemia virus type I infection in various recipients of transplants from the same donor0 aHuman Tcell leukemia virus type I infection in various recipient  a37728  c42095  a1006 - 110 v753 aBACKGROUND: The human T-cell lymphotrophic virus (HTLV) causes adult T-cell leukemia-lymphoma, tropical spastic paraparesis-HTLV type I, and associated myelopathy. METHODS: An analysis was performed of serum samples from a multiorgan donor and the five recipients. Also studied was the donor's family and the partner of one of the renal recipients. Serologic detection of anti-HTLV antibodies was carried out by enzyme immunoassay and Western blot to confirm and discriminate between HTLV types. Analysis of proviral DNA was performed by polymerase chain reaction and sequenced in the long terminal repeat region and the env gene. Peripheral blood mononuclear cell samples from all the recipients of the HTLV-I-positive organs and the donor's mother were studied. RESULTS: Two years after transplantation, three organ recipients positive for antibodies to HTLV-I were detected (two kidney transplants and one liver). All the recipients' serum samples were negative at the time of transplantation except those from the multiorgan donor. The donor's mother was born in Venezuela and was confirmed positive for antibodies to HTLV-I. The remaining family members were negative. HTLV-I DNA sequences were recovered, amplified, and sequenced from all the samples from the HTLV-I-positive recipients and the donor's mother. The homology of HTLV-I sequences was 100% in all cases. CONCLUSIONS: The authors are reporting the first documented case of HTLV-I infection in several transplant recipients sharing the same donor. The donor was infected by vertical transmission. HTLV-I infection has devastating consequences for some immunocompromised organ recipients. This emphasizes the need for a systematic survey of HTLV antibodies in all potential donors.10a*Infectious Disease Transmission, Vertical10a*Tissue Donors10aAdult10aAntibodies, Viral / analysis10aDNA, Viral / analysis10aFemale10aHTLV-I Infections / immunology / *transmission / virology10aHuman T-lymphotropic virus 1 / genetics / immunology10aHumans10aKidney Transplantation / *adverse effects10aLiver Transplantation / *adverse effects10aParaparesis, Tropical Spastic / etiology1 aGonzalez-Perez, M P1 aMunoz-Juarez, L1 aCardenas, F C1 aZarranz Imirizaldu, J J1 aCarranceja, J C1 aGarcia-Saiz, A  uhttp://613633.bywedr.asia/reference/595