03944nas a2200421   4500008004100000022004200041245016400083210006900247250001000316260000800326300001400334490000700348520250300355653004502858653001902903653001002922653003202932653001502964653002202979653007603001653001503077653004703092653001103139653003603150653003703186653003403223653005803257653001103315653004203326653001703368100001803385700001403403700001303417700001503430700001403445700001803459856004503477       1996                        eng d  a0041-1132 (Print) 0041-1132 (Linking)00aChanges in blood counts after the administration of granulocyte-colony-stimulating factor and the collection of peripheral blood stem cells from healthy donors0 aChanges in blood counts after the administration of granulocytec  a35247  cJul  a596 - 6000 v363 aBACKGROUND: After the collection of granulocyte-colony-stimulating factor (G-CSF)-mobilized peripheral blood stem cells from healthy donors, the donor platelet counts fall. However, the magnitude and duration of this decrease are not known. STUDY DESIGN AND METHODS: Sixty healthy people were given G-CSF (5, 7.5, or 10 micrograms/kg/day) for 5 days (Days 1-5), and 1 peripheral blood stem cell component was collected on Day 6. The platelet count, white cell count, absolute neutrophil count, hematocrit, and red cell count were measured before administration of G-CSF (Day 0), before collection of peripheral blood stem cells on Day 6, and on Days 8, 10, 13, 16, and 20. RESULTS: The platelet count fell from 261 +/- 47 x 10(9) cells per L on Day 0 to 159 +/- 30 x 10(9) cells per L on Day 8 (p < 0.0001) and reached its lowest level on Day 10 (146 +/- 30 x 10(9)/L; p < 0.001). Compared to Day 0 levels, the platelet count was lower on Day 13 (185 +/- 49 x 10(9)/L, p < 0.001), was the same on Day 16 (270 +/- 53 x 10(9)/L), and was greater on Day 20 (333 +/- 60 x 10(9)/L, p < 0.0001). The white cell count returned to pretreatment values on Day 13, and the absolute neutrophil count returned to pretreatment values on Day 10 (Day 0 white cell count = 6.05 +/- 1.59 x 10(9)/L and Day 0 absolute neutrophil count = 3.97 +/- 1.52 x 10(9)/L). On Day 20, both were less than pretreatment values (white cell count = 5.14 +/- 1.24 x 10(9)/L, p = 0.0007 and absolute neutrophil count = 3.20 +/- 1.24 x 10(9)/L, p = 0.0036). The red cell counts on Day 16 (4.52 +/- 0.41 x 10(12)/L) and Day 20 (4.42 +/- 0.39 x 10(12)/L) were less than Day 0 values (4.73 +/- 0.43 x 10(12)/L, p = 0.008 and p < 0.0001, respectively). The hematocrit on Day 20 (39.2 +/- 3.2%) was also less than that on Day 0 (41.2 +/- 4.8%; p = 0.01). The changes in these blood counts were not affected by the dose of the G-CSF. CONCLUSION: After stimulation with granulocyte-colony-stimulating factor and the collection or peripheral blood stem cells, the platelet counts in normal donors were decreased for at least 7 days (Days 6-13). Two weeks after collection of peripheral blood stem cells (Day 20), platelet production was increased, but the production of neutrophils and red cells was decreased. If two or more peripheral blood stem cell components are collected, then the platelet count should be measured after the second and subsequent collections. Further studies on the long-term effect of G-CSF on blood counts are needed.10a*Hematopoietic Stem Cell Transplantation10a*Tissue Donors10aAdult10aAspirin / contraindications10aFilgrastim10aFollow-Up Studies10aGranulocyte Colony-Stimulating Factor / adverse effects / *pharmacology10aHematocrit10aHemorrhagic Disorders / chemically induced10aHumans10aLeukocyte Count / *drug effects10aNeutropenia / chemically induced10aPlatelet Count / drug effects10aRecombinant Proteins / adverse effects / pharmacology10aSafety10aThrombocytopenia / chemically induced10aTime Factors1 aStroncek, D F1 aClay, M E1 aSmith, J1 aIlstrup, S1 aOldham, F1 aMcCullough, J  uhttp://613633.bywedr.asia/reference/1478