02943nas a2200241   4500008004100000020001400041024001700055245014600072210006900218260031200287300001600599490000700615520188300622100001702505700001802522700002602540700001902566700001102585700002302596700002002619700001702639856004502656       2010                        eng d  a0041-1132  awdn, 041736000aParvovirus B19 infection transmitted by transfusion of red blood cells confirmed by molecular analysis of linked donor and recipient samples.0 aParvovirus B19 infection transmitted by transfusion of red blood  aCopyright (C) 2010 Blackwell Publishing Ltd.bFrom the Division of Hematology, Center for Biologics Evaluation and Research (CBER), FDA, and the Department of Transfusion Medicine, Warren Grant Magnuson Clinical Center, NIH, Bethesda, Maryland; and the Division of Laboratory Medicine, Children's Natic2010  a1712 - 17210 v503 aBACKGROUND: Extremely high viremic levels of parvovirus B19 (B19V) can be found in acutely infected, but asymptomatic donors. However, reports of transmission by single-donor blood components are rare. In this prospective study, paired donor-recipient samples were used to investigate the transfusion risk., STUDY DESIGN AND METHODS: Posttransfusion plasma or blood samples from recipients were tested for B19V DNA by polymerase chain reaction, generally at 4 and 8 weeks, and for anti-B19V immunoglobulin (Ig)G by enzyme immunoassay, at 12 and 24 weeks. To rule out infection unrelated to transfusion, pretransfusion samples and linked donor's samples for each B19V DNA-positive recipient were assayed for B19V DNA and anti-B19V IgG and IgM. To confirm transmission, sequencing and phylogenetic analysis were performed., RESULTS: A total of 14 of 869 (1.6%) recipients were B19V DNA positive, but only 1 of 869 (0.12%; 95% confidence interval, 0.0029%-0.6409%) was negative for B19V DNA and anti-B19V IgG before transfusion and seroconverted posttransfusion. This newly infected patient received 5 x 1010 IU B19V DNA in one red blood cell (RBC) unit from an acutely infected anti-B19V-negative donor in addition to RBCs from three other donors that cumulatively contained 1320 IU of anti-B19V IgG. DNA sequencing and phylogenetic analysis showed that sequences from the linked donor and recipient were identical (Genotype 1), thus establishing transfusion transmission., CONCLUSIONS: The 0.12% transmission rate documented here, although low, could nonetheless result in hundreds or thousands of infections annually in the United States based on calculated confidence limits. Although most would be asymptomatic, some could have severe clinical outcomes, especially in neonates and those with immunocompromised or hemolytic states., Copyright (C) 2010 Blackwell Publishing Ltd.1 aYu, Mei-ying1 aAlter, Harvey1 aVirata-Theimer, Maria1 aGeng, Yansheng1 aMa, Li1 aSchechterly, Cathy1 aColvin, Camilla1 aLuban, Naomi  uhttp://613633.bywedr.asia/reference/4083